"Evolutionary conserved transcriptional response toward BMP signaling in developing mouse and xenopus embryos"
Ken Cho, Ph.D.
Professor and Chair
Department of Developmental and Cell Biology
University of California Irvine
102 Jones Hall
Carmel Bridgeman, post-doc
Dana Liu, lab technician
Immunocytochemistry antibody troubleshooting. Maintenance of KChIP transgenic mouse colony. Gonadectomy surgeries. Lab supply maintenance.
Chantelle Ferland, PhD Student
"My research focuses on epigenetic changes in response to chronically stressful stimuli and whether or not high circulating levels of glucocorticoids seen with CVS contribute to the observed pattern of chromatin remodeling. The central hypothesis of my research is that CVS, through it's dysregulation of the HPA axis and induction of high circulating levels of corticosterone results in a distinct pattern of epigenetic regulation in the hippocampus and that this pattern is responsible for the down regulation of genes, contributing to the pathology of depression. The molecular techniques that I utilize include Western Blotting, Electron Mobility Shift Assay (EMSA), Chromatin Immunoprecipitation (ChIP), RT-PCR, and IHC."
Steven Springer, PhD Student
"My research investigates a role for PI3 Kinase in the regulation of hippocampal excitability. PI3K have been shown to be important for insertion of several ion channel families, including BK channels and AMPA receptors. My research seeks to characterize which ion channel families mediate the PI3K-sensitive current. Other ongoing projects include quantifying the contribution of PI3K to LTP, assessment of changes in the afterhyperpolarization post LTP-induction, and inquiry into how several families of potassium channels set the optimal input/output frequency of the cell."
Tumay Tunur, PhD Student
"The hippocampus is a structure that plays an important role in learning and memory. Although researchers have been investigating the effects of estrogen on brain function for over 50 years, the molecular mechanism by which estrogen acts within the hippocampus is still unclear. There are, however, some candidate molecules that might play a role in downstream mechanisms of estrogen action. K+ Channel Interacting Protein 3 (KChIP3) is known to differentially modulate hippocampus-dependent memory in mice, depending on gender. It has been shown that estrogen regulates Kv4.2 surface expression. My research examines the effect of estrogen on learning strategies in mice. I am also investigating if estrogen regulates KChIP3/ Kv4.2 interaction and if this could be a possible mechanism for rapid estrogen modulation in the hippocampus."
Techniques Used: Neuro cultures, behavioral testing (place vs. response water maze), and Western blotting.
Claire Stelly, lab technician
James Irving, lab technician
Emily Evans, undergraduate
Cell and Molecular Biology, 2000 Percival Stern Hall, New Orleans, LA 70118 504-865-5546 email@example.com