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Hypertension & Renal Center of Excellence
1430 Tulane Avenue
New Orleans, LA 70112
Phone: (504) 988-3703
Fax: (504) 988-2675

 

Molecular & imaging Core


4. RIA and Chemistry Core Labs
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Core Facility Contact Information:

4. RIA and Chemistry Core Labs
These two labs allow for the quantification of various parameters from various tissues, like plasma, tissues, and urine. Techniques include:

  • HPLC separation of various angiotensin peptides from plasma and tissue extracts,
  • Detection of radioactivity signal from specific antibody-isotope-labelled ligand complexes,
  • Assessment of millimolar concentrations of Na+ and K+ in plasma and urine,
  • Measurement of millimole/kg concentrations of solutes in plasma and urine samples,
  • Quantification of fluorescence signals from ATP assays and Luciferase assays, and
  • Calculation of concentrations of unknowns for a given enzyme-linked immunoanalysis assay.

Some of the major pieces of equipment include:

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FYI: The following is brief description of some major equipment:

A) Thermo Scientific's NanoDrop 2000
Thermo Scientific's NanoDrop 2000 is a UV/Vis spectrometer that allows for the quantification of DNA, RNA, protein and peptide samples. Because it needs only 0.5 ul of sample for measurement, we can save the volume of samples. Concentration measurement capability is from 2 to 15,000 ng/ul (dsDNA), which eliminates the need to dilute samples. The measurement time is less than 5 seconds to each sample.

B) Fisher Scientific's accuSpin Micro17R Microcentrifuge
Fisher Scientific's accuSpin Micro17R Microcentrifuge is a refrigerated microcentrifuge. It can centrifuge 1.5 ml or 2.0 ml tubes up to 24 at a time. The cooling system preserves the sample quality.

Tulane University, New Orleans, LA 70118 504-865-5000 website@tulane.edu