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Hypertension & Renal Center of Excellence
1430 Tulane Avenue
New Orleans, LA 70112
Phone: (504) 988-3703
Fax: (504) 988-2675

 

Molecular & imaging Core

Molecular, Imaging, & Analytical Core Facility

Contact Information
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The Molecular, Imaging, and Analytical Core facility contributes greatly to the research projects conducted by the THRCE & COBRE investigators and provides a comprehensive service for molecular biology, imaging and biochemistry support. This facility serves as the resource to instruments and equipment needed to perform advanced molecular biology, semi-quantitative immuno-histochemistry and bio-analytical experiments, and support training of junior faculty, postdoctoral fellows, graduate and medical students in the areas of physiology, hypertension and cardiovascular and renal diseases.

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FACILITIES & RESOURCES

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The Molecular and Imaging Core Laboratory consists of the following laboratories:


1. Main laboratory


1-1. DNA workspace
This space is dedicated for DNA work such as genotyping of gene-targeted mice and analyzing for promoter regions of target genes. A well-trained laboratory research technician handle samples. He/She will extract genomic DNA from the snipped tails of gene-targeted mice and perform PCR. He/She will also set up agarose gel electrophoresis and report the final results to the corresponding investigators.


1-2. Western blot area
This space is dedicated for Western blot analysis. A well-trained laboratory research technician will handle samples. He/She will extract protein samples from human/rats/mouse samples or cells provided by the investigators. He/She will also set up polyacrylamide gel electrophoresis and report the final results to the corresponding investigators.


1-3. Shared open space
This open space is shared as a pipetting station.


1-4. Histology/Immunohistochemistry workspace
This space is dedicated for histology/immunohistochemistry works. A well-trained laboratory research technician will handle samples. He/She will set up slide glasses of human/rats/mouse samples provided by the investigators. He/She will also perform histology and/or immunohistochemistry staining and return the stained slides to the corresponding investigators.


1-5. ELISA station
This space is dedicated for ELISA work. A well-trained laboratory research technician will handle samples. He/She will prepare protein samples for ELISA from human/rats/mouse samples provided by the investigators. He/She will also set up ELISA plates and report the final results to the corresponding investigators.


1-6. RNA workspace
This space is dedicated for RNA work such like RNA extraction and real-time RT-PCR. A well-trained laboratory research technician will handle samples. He/She will extract total RNA samples from human/rats/mouse samples or cells provided by the investigators using Qiagen’s robotic system. He/She will also set up real-time RT-PCR and report the final results to the corresponding investigators.


1-7. Analytical equipment station
This space is dedicated for analytical equipments.

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2. Cell culture workroom
A separated room is dedicated for cell cultures.

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3. Microscope Workroom
A separate room dedicated for microscope and image analyses.

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4. RIA and Chemistry Core Labs
These two labs allow for the quantification of various parameters from various tissues, like plasma, tissues, and urine. 

Tulane University, New Orleans, LA 70118 504-865-5000 website@tulane.edu