The DNA Sequencing Core at the Center for Stem Cell Research and Regenerative Medicine, Tulane University Health Sciences Center operates two ABI Prism 3130xl sequencers. Compared to the gel-based sequencing systems, the advantages of the capillary sequencer include a lower error rate (automated sample loading, gel/polymer issues virtually non-existent), higher through-put and reduced cost of operation. We have 10 years of experience in running a middle-throughput DNA sequencing core and are committed to helping in-house researchers in all aspects of the sequencing (primer design, template purification, data analysis), not only in running the samples.
With the best samples we can achieve >750-bp read-lengths but the error rate starts to get significantly higher after ~650-bp. With these two instruments we can process about 384 samples a day depending in the read-length.
Pricing InformationThe charge per reaction is $15. If the reactions are brought in 96 reaction- sets in 96-well format PCR-plate, the charge per reaction is $10.
Sample PreparationAll samples must be submitted with a sequencing requisition form. An IT must be submitted at the beginning of each month.
PCR ProductRemove the remaining primers and dNTPs from the PCR reaction with your favorite purification method. Prepare the reactions in a 0.2 ml PCR reaction tube using the following guidelines:
PlasmidPurify the plasmid DNA with your favorite purification method. The cleanliness of the sample is paramount in plasmid sequencing. Prepare the reactions in a 0.2 ml PCR reaction tube using the following guidelines: