Faculty » Research Information
Y-Teh Li, PhD
Department of Biochemistry and Molecular Biology
Tulane University School of Medicine
1430 Tulane Avenue, #8543
New Orleans, LA, 70112
Glycobiology – Biomedically useful glycosidases and biochemical basis of inborn lysosomal diseases
Y.-T. Li and his wife, S.-C. Li, have been working as a team in the field of glycobiology for over three decades. The two major projects of their laboratory are: (i) biochemical studies of inborn lysosomal diseases and (ii) studies of biomedically useful glycosidases. S.-C. Li is in charge of the first project (i) and Y.-T. Li is responsible for the second project (ii).
The long-standing interests of the biomedically useful glycosidases project in the Li's laboratory have been centered on the identification, isolation, cloning and characterization of unique and biomedically useful glycosidases, and to make them available to other investigators. They have discovered several useful glycosidases and have contributed richly to the use of glycosidases to study the structure, function and catabolism of glycoconjugates. Their laboratory was the first to use jack bean alpha-mannosidase to show the presence of alpha-mannosidic linkage in glycoproteins (J. Biol. Chem. 241, 1010, '66). Several commercially available glycosidases were developed based on the methods established in their laboratory. Their representative glycoconjugate-cleaving enzymes include: jack bean beta-hexosaminidase (J. Biol. 245, 5153, '70); pineapple alpha- and beta-mannosidases (J. Biol. 247, 3677, '72); jack bean beta-galactosidae (J. Biol. 250, 6786, '75); limpet alpha-N-acetylgalactosaminidase (J. Biol. 252, 5194, '77); Escherichia freundii endo-beta-galactosidase (J. Biol. 255, 5955, '80); ceramide glycanase that cleaves the linkage between the ceramide and the glycane chain in glycosphingolipids (J. Biol. 264, 12272, '89); NeuAc-alpha-2,3-Gal-specific sialidase (J. Biol. Chem. 271, 19219, '96); alpha-KDOase (J. Biol. Chem. 272, 26419, '97); alpha-KDN-sialidases (J. Biol. Chem. 274, 31974, '99); an endo-beta-galactosidase that liberates the disaccharide GlcNAc-alpha-1,4-Gal from glycans specifically expressed in gastric gland mucous cell-type mucin (J. Biol. Chem. 276, 28226 '01, Biochemistry 41, 2388, '02); an endo-beta-galactosidase that cleaves both blood group A and B glycotopes (J. Biol. Chem. 280, 7720, '05). They have been supported by the same grant from the NIH for 36 years. Y.-T. Li was a twice recipient of the Javits Neuroscience Investigator Award from the NIH.
Anderson, K., Ashida, H., Maskos, K., Dell, A., Li, S.-C., and Li, Y.-T. (2005) A clostridial endo-beta-galactosidase that cleaves both blood group A and B glycotopes: the first member of a new glycoside hydrolase family GF98. J. Biol. Chem. 280, 7720-7728.
Ando, T., Li, S.-C., Ito, M., and Li Y.-T. (2005) Facile method for the preparation of lyso-GM1 and lyso-GM2. J. Chromatogr. A 1078, 193-195.
Memcarelli, S., Cavalieri, C., Magini, A., Tancini, B., Basso, L., Lemansky, P., Hasilik, A., Li, Y.-T., chigoro, V., Emiliani, C., and Sonnino, S. (2005) Identification of plasma membrane associated mature beta-hexosaminidase A active towards GM2 ganglioside, in human fibroblasts. FEBS Lett. 579, 5501-5506.
Arfi, A., Bourgoin, C., Basso, L., Emiliani, C., Tanicini, B., Chigorno, V., Li, Y.-T.,Orlacchio, A., Poenaru, L., Sonnino, S., and Caillaud, C. (2005) Bicistronic lentiviral vector corrects beta-hexosaminidase deficiency in transduced and cross-corrected human Sandhoff fibroblasts. Neurobiol Dis. 20, 583-593.
Fuse, T., Ando, H., Imamura, A., Sawada, N., Ishida, H., Kiso, M., Ando, T., Li, S.-C., and Li, Y.-T. (2006) Synthesis and enzymatic susceptibility of a series of novel GM2 analogs. Glycoconj. J. 23, 329-343.
Nakagawa, H., Hama., Y., Sumi, T., Li, S.-C., Masko, K., Kalayanamitra, K., Mizumoto, S., Sugahara, K., and Li, Y.-T. ( 2007) Occurrence of a non-sulfated chondroitin proteoglycan in the dried saliva of collocalia swiftlets (edible bird's nest). Glycobiology 17, 157-162.
Kobayashi, K., Liu, D., Ogawa, H., Miwa, Y., Nagasaka, T., Maruyama, S., Li, Y.-T., Onishi, A., Kuzuya, T., Kadomatsu, K., Uchida, K., and Nakao, A. (2007) Alternative strategy for overcoming ABO incompatibility. Transplantation 83, 1284-1286.
Ginzburg, L., Li, S.-C., Li, Y.-T. and Futerman, A. H. (2008) An exposed carboxyl group on sialic acid is essential for ganglioside to inhibit calcium uptake via the sarco/endoplastic reticulum Ca2+-ATPase: relevance to gangliosidoses. J. Neurochem. 104, 140-146.
Li, Y.-T., Li, S.-C., Kiso, M., Ishida, H., Mauri, L., Raimondi, L., Bernardi, A., and Sonnino, S. (2008) Effect of structural modification of ganglioside GM2 on intra-molecular and inter-molecular carbohydrate-to-carbohydrate interaction and enzymatic susceptibility. Biochim. Biophys. Acta 1780, 353-361.
Komori, T., Ando, T., Imamura, A., Li, Y-T., Ishida, H., and Kiso, M. (2008) Design and efficient synthesis of novel GM2 analogues with respect to the elucidation of the function of GM2 activator. Glycoconj. J. 25, 647-661.
Goto-Inoue, N., Hayasaka, T., Sugiura, Y., Taki, T., Li, Y.-T., Matsumoto, M., and Setou, M. (2008) High-sensitivity analysis of glycosphingolipids by matrix-assisted lasser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes. J. Chromatogr. B 870, 74-83.
Kobayashi, T., Liu, D., Ogawa, H., Nagasaka, T., Maruyama, S., Li, Y.-T., Onishi, A., Iwamoto, M., Kzuya, T., Kadomatsu, K., Uchida, K., and Nakao, A. (2009) Removal of blood group A/B antigen by ex vivo administration of endo-beta-galactosidase (ABase) for ABO-incompatible transplantation. Transplant Immunology 20, 132-138.
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