Faculty » Research Information
Samuel J. Landry, PhD
Department of Biochemistry and Molecular Biology
Tulane University School of Medicine
1430 Tulane Avenue, #8543
New Orleans, LA 70112
Research Interests/Area of Student: Protein folding and protein-protein interactions; molecular chaperone proteins; antigen processing.
Summary: Our work employs biochemical and biophysical techniques to investigate structure/function relationships in protein-protein interactions, especially those involved in cellular stress and immune responses.
Mechanism of Hsp60/Hsp10 Chaperonin Specificity: Chaperonins are a class of stress-induced molecular chaperones characterized by a ring-shaped oligomeric structure and the distinctive ability to use ATP to promote protein folding. Our studies aim to illuminate mechanistic features of chaperonin function and identify determinants of Hsp60 specificity for substrates and Hsp10s.
E. coli DnaJ/DnaK: Members of the Hsp40 and Hsp70 families of chaperones are implicated in a host of cellular processes in all organisms including DNA replication, gene expression, and protein translocation across membranes, in addition to the stress response. The goal of this research is to understand at a physical chemical level how Escherichia coli molecular chaperones DnaJ (an Hsp40) and DnaK (an Hsp70) cooperate in the management of protein-protein interactions.
Structural Instability and T-cell Epitope Immunodominance: Humoral and cellular immunity to HIV and other pathogens depend on antigen-specific CD4+ T cell responses directed against peptides presented in MHC class II antigen presenting proteins. Generally, CD4+ T-cell responses are focused on only a few immunodominant regions of naturally processed antigens. We have found that immunodominant regions occur in the sequences flanking locally unstable segments of an antigen's three-dimensional structure. We are now modifying CD4+ helper T-cell immune responses to recombinant antigens by engineering their processing and presentation, and studying how the specificity of the CD4+ T-cell response influences the effector phases of the immune response.
Tulane University, New Orleans, LA 70118 504-865-5000 email@example.com